| 质粒名称: | pQFlag-USP7 WT puroR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46751 |
| 存储实验室: | Goedele Maertens, Gordon Peters |
|
载体骨架基本信息 |
|
| 载体名称: | pQCXIP |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 10491 bp |
| 载体修饰: | NLS（插入物上的C末端）pQFlag-puro通过连接在NotI-和BamHI消化的pQCXIP中进行工程化，产生以下MC:NotI Kozak MfeI Flag AgeI XhoI BamHI |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | USP7 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | Flag（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | CMV Forward |
| 3' 测序引物 | pCDH-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pQFlag-USP7 WT puroR was a gift from Goedele Maertens & Gordon Peters (Addgene plasmid # 46751 ; http://n2t.net/addgene:46751 ; RRID:Addgene_46751) |
| 参考文献部分: | Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G. EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub 2010 Jul 2. 10.1038/emboj.2010.129 PubMed 20601937 |
买家导航
pQFlag-USP7 WT puroR载体质粒基本信息
pQFlag-USP7 WT puroR质粒图谱载体图谱和pQFlag-USP7 WT puroR载体序列质粒序列多克隆位点信息
pQFlag-USP7 WT puroR质粒载体简介
pQFlag-USP7 WT puroR质粒序列载体序列
LOCUS pQFlag-USP7 WT puroR 10495 bp ds-DNA circular SYN 11-JUN-2019
DEFINITION Retroviral vector that expresses wild type Flag-tagged human USP7.
ACCESSION .
VERSION .
KEYWORDS pQFlag-USP7 WT puroR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10495)
AUTHORS Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G
TITLE Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation
of the INK4a tumour suppressor.
JOURNAL EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub
2010 Jul 2.
PUBMED 20601937
REFERENCE 2 (bases 1 to 10495)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 11, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10495
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 309..512
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 513..688
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 751..950
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1151..1567
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1497..1519
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1535..1551
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
enhancer 1604..1907
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1908..2111
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2108..2132
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2268..2291
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
misc_feature 5622..6195
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5789..5806)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 6016..6035
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 6228..6827
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6228..6247)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6724..6744
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 7201..7626
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
Moloney murine leukemia virus"
primer_bind complement(7729..7751)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7887..7907)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7892..8221
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8072..8207
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8134..8153
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 8243..8262
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(8343..8360)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8514..9102)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8594..8613)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9273..10133)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9896..9915
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10134..10238)
/gene="bla"
/label=AmpR promoter
primer_bind 10306..10324
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
enhancer 10424..308
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
2221 cggacctgca ggaattgatc cgcggccgcg acaccatggc acaattggat tacaaggatg
2281 acgatgacaa gaccggtatg aaccaccagc agcagcagca gcagcagaaa gcgggcgagc
2341 agcagttgag cgagcccgag gacatggaga tggaagcggg agatacagat gacccaccaa
2401 gaattactca gaaccctgtg atcaatggga atgtggccct gagtgatgga cacaacaccg
2461 cggaggagga catggaggat gacaccagtt ggcgctccga ggcaaccttt cagttcactg
2521 tggagcgctt cagcagactg agtgagtcgg tccttagccc tccgtgtttt gtgcgaaatc
2581 tgccatggaa gattatggtg atgccacgct tttatccaga cagaccacac caaaaaagcg
2641 taggattctt tctccagtgc aatgctgaat ctgattccac gtcatggtct tgccatgcac
2701 aagcagtgct gaagataata aattacagag atgatgaaaa gtcgttcagt cgtcgtatta
2761 gtcatttgtt cttccataaa gaaaatgatt ggggattttc caattttatg gcctggagtg
2821 aagtgaccga tcctgagaaa ggatttatag atgatgacaa agttaccttt gaagtctttg
2881 tacaggcgga tgctccccat ggagttgcgt gggattcaaa gaagcacaca ggctacgtcg
2941 gcttaaagaa tcagggagcg acttgttaca tgaacagcct gctacagacg ttatttttca
3001 cgaatcagct acgaaaggct gtgtacatga tgccaaccga gggggatgat tcgtctaaaa
3061 gcgtcccttt agcattacaa agagtgttct atgaattaca gcatagtgat aaacctgtag
3121 gaacaaaaaa gttaacaaag tcatttgggt gggaaacttt agatagcttc atgcaacatg
3181 atgttcagga gctttgtcga gtgttgctcg ataatgtgga aaataagatg aaaggcacct
3241 gtgtagaggg caccataccc aaattattcc gcggcaaaat ggtgtcctat atccagtgta
3301 aagaagtaga ctatcggtct gatagaagag aagattatta tgatatccag ctaagtatca
3361 aaggaaagaa aaatatattt gaatcatttg tggattatgt ggcagtagaa cagctcgatg
3421 gggacaataa atacgacgct ggggaacatg gcttacagga agcagagaaa ggtgtgaaat
3481 tcctaacatt gccaccagtg ttacatctac aactgatgag atttatgtat gaccctcaga
3541 cggaccaaaa tatcaagatc aatgataggt ttgaattccc agagcagtta ccacttgatg
3601 aatttttgca aaaaacagat cctaaggacc ctgcaaatta tattcttcat gcagtcctgg
3661 ttcatagtgg agataatcat ggtggacatt atgtggttta tctaaacccc aaaggggatg
3721 gcaaatggtg taaatttgat gacgacgtgg tgtcaaggtg tactaaagag gaagcaattg
3781 agcacaatta tgggggtcac gatgacgacc tgtctgttcg acactgcact aatgcttaca
3841 tgttagtcta catcagggaa tcaaaactga gtgaagtttt acaggcggtc accgaccatg
3901 atattcctca gcagttggtg gagcgattac aagaagagaa aaggatcgag gctcagaagc
3961 ggaaggagcg gcaggaagcc catctctata tgcaagtgca gatagtcgca gaggaccagt
4021 tttgtggcca ccaagggaat gacatgtacg atgaagaaaa agtgaaatac actgtgttta
4081 aagtattgaa gaactcctcg cttgctgagt ttgttcagag cctctctcag accatgggat
4141 ttccacaaga tcaaattcga ttgtggccca tgcaagcaag gagtaatgga acaaaacgac
4201 cagcaatgtt agataatgaa gccgacggca ataaaacaat gattgagctc agtgataatg
4261 aaaacccttg gacaatattc ctggaaacag ttgatcccga gctggctgct agtggagcga
4321 ccttacccaa gtttgataaa gatcatgatg taatgttatt tttgaagatg tatgatccca
4381 aaacgcggag cttgaattac tgtgggcata tctacacacc aatatcctgt aaaatacgtg
4441 acttgctccc agttatgtgt gacagagcag gatttattca agatactagc cttatcctct
4501 atgaggaagt taaaccgaat ttaacagaga gaattcagga ctatgacgtg tctcttgata
4561 aagcccttga tgaactaatg gatggtgaca tcatagtatt tcagaaggat gaccctgaaa
4621 atgataacag tgaattaccc accgcaaagg agtatttccg agatctctac caccgcgttg
4681 atgtcatttt ctgtgataaa acaatcccta atgatcctgg atttgtggtt acgttatcaa
4741 atagaatgaa ttattttcag gttgcaaaga cagttgcaca gaggctcaac acagatccaa
4801 tgttgctgca gtttttcaag tctcaaggtt atagggatgg cccaggtaat cctcttagac
4861 ataattatga aggtacttta agagatcttc tacagttctt caagcctaga caacctaaga
4921 aactttacta tcagcagctt aagatgaaaa tcacagactt tgagaacagg cgaagtttta
4981 aatgtatatg gttaaacagc caatttaggg aagaggaaat aacactatat ccagacaagc
5041 atgggtgtgt ccgggacctg ttagaagaat gtaaaaaggc cgtggagctt ggggagaaag
5101 catcagggaa acttaggctg ctagaaattg taagctacaa aatcattggt gttcatcaag
5161 aagatgaact attagaatgt ttatctcctg caacgagccg gacgtttcga atagaggaaa
5221 tccctttgga ccaggtggac atagacaaag agaatgagat gcttgtcaca gtggcgcatt
5281 tccacaaaga ggtcttcgga acgttcggaa tcccgttttt gctgaggata caccagggcg
5341 agcattttcg agaagtgatg aagcgaatcc agagcctgct ggacatccag gagaaggagt
5401 ttgagaagtt taaatttgca attgtaatga tgggccgaca ccagtacata aatgaagacg
5461 agtatgaagt aaatttgaaa gactttgagc cacagcccgg taatatgtct catcctcggc
5521 cttggctagg gctcgaccac ttcaacaaag ccccaaagag gagtcgctac acttaccttg
5581 aaaaggccat taaaatccat aactgaggat ccggaattcc gcccccctcc ctcccccccc
5641 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
5701 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
5761 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
5821 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
5881 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
5941 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
6001 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
6061 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
6121 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
6181 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca
6241 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
6301 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
6361 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
6421 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
6481 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
6541 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
6601 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
6661 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
6721 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
6781 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
6841 acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga agccacccgg
6901 ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagtc gagggctgca
6961 gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt ccgagggaca
7021 ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc aatttgttaa
7081 agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc
7141 tatagagtac gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg
7201 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
7261 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
7321 agggtcgacc ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
7381 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
7441 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
7501 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
7561 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
7621 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
7681 cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
7741 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
7801 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
7861 cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
7921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
7981 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
8041 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
8101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
8161 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
8221 aaagcttact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
8281 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
8341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
8401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
8461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
8521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
8581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
8641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
8701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
8761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
8821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
8881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
8941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
9001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
9061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
9121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
9181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
9241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
9301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
9361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
9421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
9481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
9541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
9601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
9661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
9721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
9781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
9841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
9901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
9961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
10021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
10081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
10141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
10201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
10261 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
10321 aggccctttc gtcttcaaga attagcttgg ccattgcata cgttgtatcc atatcataat
10381 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
10441 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagt
//
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